Regulatory

Part:BBa_K4451024

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


pET-21a(+) T7-LacO + RBS

BBa_K4451024 is the IPTG-inducible T7 promoter and ribosome binding site from pET-21a+ (Novagen 69740).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None